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renilla prl sv40p plasmid  (Addgene inc)


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    Structured Review

    Addgene inc renilla prl sv40p plasmid
    Renilla Prl Sv40p Plasmid, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 119 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/renilla+prl+sv40p+plasmid/pRL-SV40P+(Plasmid+%2327163)/pm41935049-280-38-44
    Average 93 stars, based on 119 article reviews
    renilla prl sv40p plasmid - by Bioz Stars, 2026-10
    93/100 stars

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    Related Articles

    Activity Assay:

    Article Title: Inhibition of circulating glycocholic acid-regulated signaling potentiates immune checkpoint therapy in colorectal cancer.
    Article Snippet: .. For measurement of FXR activity, cells were seeded into 24-well plates at ~1.5x104 cells per well and transfected with the FXR luciferase reporter plasmid FXRE-Luc (200 ng; cat. no. 11764ES03; Shanghai Yeasen Biotechnology Co., Ltd.) and 20 ng Renilla pRL-SV40P plasmid (cat. no. 27163; Addgene, Inc.) per well with Lipofectamine® 3000 according to the manufacturer’s instructions. .. Next, cells were treated with GCA (100nM, cat. no. G2878; Sigma‐Aldrich; Merck KGaA) or GW4064 (5μM, cat. no. G5172; Sigma‐Aldrich; Merck KGaA) for 24 h, and the relative luciferase units (RLUs) were measured using the Dual-Glo Luciferase Assay System (Promega Corporation) according to the manufacturer’s instructions.

    Transfection:

    Article Title: Inhibition of circulating glycocholic acid-regulated signaling potentiates immune checkpoint therapy in colorectal cancer.
    Article Snippet: .. For measurement of FXR activity, cells were seeded into 24-well plates at ~1.5x104 cells per well and transfected with the FXR luciferase reporter plasmid FXRE-Luc (200 ng; cat. no. 11764ES03; Shanghai Yeasen Biotechnology Co., Ltd.) and 20 ng Renilla pRL-SV40P plasmid (cat. no. 27163; Addgene, Inc.) per well with Lipofectamine® 3000 according to the manufacturer’s instructions. .. Next, cells were treated with GCA (100nM, cat. no. G2878; Sigma‐Aldrich; Merck KGaA) or GW4064 (5μM, cat. no. G5172; Sigma‐Aldrich; Merck KGaA) for 24 h, and the relative luciferase units (RLUs) were measured using the Dual-Glo Luciferase Assay System (Promega Corporation) according to the manufacturer’s instructions.

    Luciferase:

    Article Title: Inhibition of circulating glycocholic acid-regulated signaling potentiates immune checkpoint therapy in colorectal cancer.
    Article Snippet: .. For measurement of FXR activity, cells were seeded into 24-well plates at ~1.5x104 cells per well and transfected with the FXR luciferase reporter plasmid FXRE-Luc (200 ng; cat. no. 11764ES03; Shanghai Yeasen Biotechnology Co., Ltd.) and 20 ng Renilla pRL-SV40P plasmid (cat. no. 27163; Addgene, Inc.) per well with Lipofectamine® 3000 according to the manufacturer’s instructions. .. Next, cells were treated with GCA (100nM, cat. no. G2878; Sigma‐Aldrich; Merck KGaA) or GW4064 (5μM, cat. no. G5172; Sigma‐Aldrich; Merck KGaA) for 24 h, and the relative luciferase units (RLUs) were measured using the Dual-Glo Luciferase Assay System (Promega Corporation) according to the manufacturer’s instructions.

    Plasmid Preparation:

    Article Title: Inhibition of circulating glycocholic acid-regulated signaling potentiates immune checkpoint therapy in colorectal cancer.
    Article Snippet: .. For measurement of FXR activity, cells were seeded into 24-well plates at ~1.5x104 cells per well and transfected with the FXR luciferase reporter plasmid FXRE-Luc (200 ng; cat. no. 11764ES03; Shanghai Yeasen Biotechnology Co., Ltd.) and 20 ng Renilla pRL-SV40P plasmid (cat. no. 27163; Addgene, Inc.) per well with Lipofectamine® 3000 according to the manufacturer’s instructions. .. Next, cells were treated with GCA (100nM, cat. no. G2878; Sigma‐Aldrich; Merck KGaA) or GW4064 (5μM, cat. no. G5172; Sigma‐Aldrich; Merck KGaA) for 24 h, and the relative luciferase units (RLUs) were measured using the Dual-Glo Luciferase Assay System (Promega Corporation) according to the manufacturer’s instructions.

    Article Title: Targeting cancer-associated fibroblast autophagy renders pancreatic cancer eradicable with immunochemotherapy by inhibiting adaptive immune resistance
    Article Snippet: Tumor cells were seeded into 96-well plates with at 50%-70% confluency and then transfected into five copies using Lipofectamine 3000 (Life Technologies, L3000–075) according to the manufacturer’s manual. .. In detail, 150 ng of each experimental plasmid (pGL4.10 empty vector, pENTER empty vector, pGL4.10-USP14, pENTER STAT3 or pGL4.10-USP14-STAT3 mutation vector [Shanghai OBiO Technology]) and 7.5 ng of the Renilla pRL-SV40P plasmid (Addgene, plasmid 27,163; deposited by Ron Prywes) used for normalization per well. ..

    Article Title: Targeting cancer-associated fibroblast autophagy renders pancreatic cancer eradicable with immunochemotherapy by inhibiting adaptive immune resistance.
    Article Snippet: Dual luciferase Reporter assay Tumor cells were seeded into 96-well plates with at 50%-70% confluency and then transfected into five copies using Lipofectamine 3000 (Life Technologies, L3000–075) according to the manufacturer’s manual. .. In detail, 150 ng of each experimental plasmid (pGL4.10 empty vector, pENTER empty vector, pGL4.10-USP14, pENTER STAT3 or pGL4.10USP14-STAT3 mutation vector [Shanghai OBiO Technology]) and 7.5 ng of the Renilla pRL-SV40P plasmid (Addgene, plasmid 27,163; deposited by Ron Prywes) used for normalization per well. ..

    Mutagenesis:

    Article Title: Targeting cancer-associated fibroblast autophagy renders pancreatic cancer eradicable with immunochemotherapy by inhibiting adaptive immune resistance
    Article Snippet: Tumor cells were seeded into 96-well plates with at 50%-70% confluency and then transfected into five copies using Lipofectamine 3000 (Life Technologies, L3000–075) according to the manufacturer’s manual. .. In detail, 150 ng of each experimental plasmid (pGL4.10 empty vector, pENTER empty vector, pGL4.10-USP14, pENTER STAT3 or pGL4.10-USP14-STAT3 mutation vector [Shanghai OBiO Technology]) and 7.5 ng of the Renilla pRL-SV40P plasmid (Addgene, plasmid 27,163; deposited by Ron Prywes) used for normalization per well. ..

    Article Title: Targeting cancer-associated fibroblast autophagy renders pancreatic cancer eradicable with immunochemotherapy by inhibiting adaptive immune resistance.
    Article Snippet: Dual luciferase Reporter assay Tumor cells were seeded into 96-well plates with at 50%-70% confluency and then transfected into five copies using Lipofectamine 3000 (Life Technologies, L3000–075) according to the manufacturer’s manual. .. In detail, 150 ng of each experimental plasmid (pGL4.10 empty vector, pENTER empty vector, pGL4.10-USP14, pENTER STAT3 or pGL4.10USP14-STAT3 mutation vector [Shanghai OBiO Technology]) and 7.5 ng of the Renilla pRL-SV40P plasmid (Addgene, plasmid 27,163; deposited by Ron Prywes) used for normalization per well. ..



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    Image Search Results


    Journal: Cell reports

    Article Title: Distinct RORγt-dependent Th17 immune responses are required for autoimmune pathogenesis and protection against bacterial infection

    doi: 10.1016/j.celrep.2024.114951

    Figure Lengend Snippet:

    Article Snippet: pRL-SV40 Renilla luciferase control , Addgene , RRID:Addgene_27163.

    Techniques: Virus, Recombinant, Modification, Protease Inhibitor, Lysis, Radio Immunoprecipitation, Isolation, cDNA Synthesis, SYBR Green Assay, Emulsion, Reporter Assay, Microarray, CRISPR, Luciferase, Control, Software